pancreatic cancer cell line panc1  (ATCC)

Journal: Biochemistry and Biophysics Reports

Article Title: Exploring the impact of hAMSCs secretome on Panc1 cells via TNF-α/NF-κB (p50/p65)/Caspase 3 signaling pathways: An in vitro study

doi: 10.1016/j.bbrep.2025.102411

Figure Lengend Snippet: Schematic model of the co-culture system used in this study (A). The induction of TNF-α expression in hAMSCs secretome-treated Panc1 cells using Western blot and an ELISA kit (B and C). Actin was used as an internal control (TCL: total cell lysate). Data represent mean ± SD of three independent experiments. ∗p < 0.05 was considered statistically significant.

Article Snippet: HAMSCs (Cat No: C10680 , human, female, newborn, spindle-shaped) were obtained from the Iranian Biological Resource Center located in Tehran, Iran, while Panc1 pancreatic cancer cells (NCBI No: C556) were acquired from the Pasteur Institute, also situated in Tehran, Iran.

Techniques: Co-Culture Assay, Expressing, Western Blot, Enzyme-linked Immunosorbent Assay, Control

Journal: Biochemistry and Biophysics Reports

Article Title: Exploring the impact of hAMSCs secretome on Panc1 cells via TNF-α/NF-κB (p50/p65)/Caspase 3 signaling pathways: An in vitro study

doi: 10.1016/j.bbrep.2025.102411

Figure Lengend Snippet: Schematic model of NF-κB activation (A). The expression of p-IKK, p-IKKα/β, NF-κB (p50/p65), IκB, and p-IκB proteins was analyzed using Western blot in hAMSCs-treated Panc1 cancer cells (B, C, and D). Actin was used as an internal control (TCL: total cell lysate). The induction of NF-κB (p50/p65) expression in hAMSCs secretome-treated Panc1 cells was measured using an ELISA kit (E and F). Data represent mean ± SD of three independent experiments. ∗p < 0.05 was considered statistically significant. N.S: No significant.

Article Snippet: HAMSCs (Cat No: C10680 , human, female, newborn, spindle-shaped) were obtained from the Iranian Biological Resource Center located in Tehran, Iran, while Panc1 pancreatic cancer cells (NCBI No: C556) were acquired from the Pasteur Institute, also situated in Tehran, Iran.

Techniques: Activation Assay, Expressing, Western Blot, Control, Enzyme-linked Immunosorbent Assay

Journal: Biochemistry and Biophysics Reports

Article Title: Exploring the impact of hAMSCs secretome on Panc1 cells via TNF-α/NF-κB (p50/p65)/Caspase 3 signaling pathways: An in vitro study

doi: 10.1016/j.bbrep.2025.102411

Figure Lengend Snippet: The induction of IL-1β and IL-8 expression in hAMSCs secretome-treated Panc1 cells was analyzed using an ELISA kit and Western blot (A–C). Data represent mean ± SD of three independent experiments. ∗p < 0.05 was considered statistically significant. Actin was used as an internal control (TCL: total cell lysate).

Article Snippet: HAMSCs (Cat No: C10680 , human, female, newborn, spindle-shaped) were obtained from the Iranian Biological Resource Center located in Tehran, Iran, while Panc1 pancreatic cancer cells (NCBI No: C556) were acquired from the Pasteur Institute, also situated in Tehran, Iran.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Western Blot, Control

Journal: Biochemistry and Biophysics Reports

Article Title: Exploring the impact of hAMSCs secretome on Panc1 cells via TNF-α/NF-κB (p50/p65)/Caspase 3 signaling pathways: An in vitro study

doi: 10.1016/j.bbrep.2025.102411

Figure Lengend Snippet: Cellular apoptosis induction in hAMSCs-treated Panc1 cancer cells using DAPI. The experiments were conducted three times (original microscope magnification, 40X, Scale bar, 10 μm) (A). Inhibitory effect of hAMSCs on Panc1 cancer cells using MTT assay. Data represent mean ± SD of three independent experiments. ∗p < 0.05 was considered statistically significant (B). The expression of p53, PUMA, and Caspase 3 proteins was shown in hAMSCs-treated Panc1 cells using Western blot (C, D). Actin was used as an internal control (TCL: total cell lysate).

Article Snippet: HAMSCs (Cat No: C10680 , human, female, newborn, spindle-shaped) were obtained from the Iranian Biological Resource Center located in Tehran, Iran, while Panc1 pancreatic cancer cells (NCBI No: C556) were acquired from the Pasteur Institute, also situated in Tehran, Iran.

Techniques: Microscopy, MTT Assay, Expressing, Western Blot, Control

Journal: Biochemistry and Biophysics Reports

Article Title: Exploring the impact of hAMSCs secretome on Panc1 cells via TNF-α/NF-κB (p50/p65)/Caspase 3 signaling pathways: An in vitro study

doi: 10.1016/j.bbrep.2025.102411

Figure Lengend Snippet: A schematic summary of the present study. HAMSCs secretome enables the induction of TNF-α, IL-1β, IL-8, p-IKK, p-IKKα, p-IKKβ, p-IκB, p53, PUMA, Caspase 3, NF-κB (p50/p65) and down-regulation of IKβ expressions in Panc1 cancer cells.

Article Snippet: HAMSCs (Cat No: C10680 , human, female, newborn, spindle-shaped) were obtained from the Iranian Biological Resource Center located in Tehran, Iran, while Panc1 pancreatic cancer cells (NCBI No: C556) were acquired from the Pasteur Institute, also situated in Tehran, Iran.

Techniques: